endonuclease造句
1. Samples were digested with the appropriate restriction endonuclease and were size fractionated by electrophoresis through 0.9% agarose gels.
2. Restrictive endonuclease site Eag I was eliminated.
3. Methods:DNA sequencing and restriction endonuclease reaction was used.
4. PCR products were digested with endonuclease and sequenced.
5. After a restriction endonuclease cleavage, two expected smaller fragments were observed.
6. The endonuclease digestion map of pWTA was consistent with the fragment length in anticipation.
7. Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.
8. Conclusion Increase in cytoplasmic calcium activates endonuclease (s) which causes DNA fragmentation at internucleosomal sites and neuronal apoptosis.
9. Uses advanced biotechnology to simulate the T4 Endonuclease produced by natural marine algae. This helps to repair and restore damaged DNA cells in the skin and erase fine lines and wrinkles.
10. The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
11. The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
12. Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
13. Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.
14. Results The result of restriction endonuclease digestion was accordance with the anticipated objective strap size .
15. Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.
16. They then introduced the homing endonuclease gene (HEG) — which makes a copy of itself, ensuring all offspring end up with a copy of it as well — into around one per cent of the population.
17. Restriction enzyme:Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
18. Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
19. Secondly, the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed DNA adapter by ligase.
20. Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.
21. CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.
22. Results:The constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
23. In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.
24. Finally, the cloned B antigen gene was identified by restriction endonuclease analysis, DIG probe hybridization and DNA sequencing.
25. The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.
26. The core technology in TILLING is screening mutant library by using single-strand endonuclease.
27. Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.
28. Bufalin induced apoptosis of HL 60 cells was inhibited by ZnCl 2, an inhibitor of endonuclease, but not by cycloheximide, an inhibitor of protein synthesis.
29. Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.