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61. The plasma prolactin(PRL) level was measured before, during, and after treatment(monthly) Serumprolactin was examined with ELISA and observe the amenorrhea and galactorrhea. 62. Methods of ELISA, nonradioactive molecular hybridization and RT PCR were applied in the detection of rice grassy stunt virus (RGSV). 63. Methods: The sputa of 30 lung cancer patients and 25 benign lung disease patients(control group) were checked for telomerase activity with PCR-TRAP ELISA. 64. Objective To prepare an ELISA kit for detecting tetanus IgG rapidly. 65. This research established a solid foundation to find new method of detecting APP such as ELISA, PCR etc and produce an Actinobacillus pleuropleumoniae genetic engineering vaccine strain effective. 66. Methods The contents of SOD, GSH-PX, LPO, MDA, CAT and TNF in the blood of infected person and patient of hepatitis were detected receptively by using ELISA and colorimetry. 67. Methods Relative sensitivity of various conjugates in ELISA for the detection of ScFv was assessed. 68. Cathepsin E , Maspin, S 100 P was analyzed with immunohistochemical analysis , PCR , ELISA, Western blotting in the specimens obtained by EUS - FNA. 69. Objective To prepare celluloseby hybridoma technique, and to establish I - ELISA method for clinical application. 70. Aim To make ELISA typing of sera from HFRS patients from partial districts of Hebei and Shaanxi Provinces using hantavirus nucleocapsid protein(NP) as antigen. 71. Methods Purify HPV16 L1 protein by electroelution and determine its immunoreactivity by double immunodiffusion test and ELISA. 72. S ELISA for detection of circulating antigen ( CAg ) of hydatid disease was established. 73. MethodsIn Tianjin 6 077 patients with hepatosis were determined for pathogeny by ELISA and RT PCR; HGV's infection state was analyzed and compared in different kinds of hepatosis. 74. Methods: Enzyme linked immunosolid assay ( ELISA ) was used to detect the level of ACA - IgG in 368 cases. 75. Method Field investigation, data collection in misdiagnosed cases, IDT(intradermal test)and ELISA for detection of susceptible persons, freshwater crab and reservoir host examination etc. 76. This indicated that dot - ELISA had high specificity and sensitivity in the early diagnosis of trichinosis. 77. Methods ELISA was used to detect FK506 valley point in 27 renal transplant patients at different postoperative periods. Based on the results combined with clinical findings, the dosages were adjusted. 78. Methods:Human basophil degranulation test (HBDT), ELISA were evaluated and compared in 30 healthy subjects and 30 asthmatics, using respectively WBE and FE. 79. Specific IgG antibody was tested by ELISA, the sonicated B . pertussis bacteria was used as coating antigen. 80. After two rounds of panning, 21 positive plaques were selected each round at random respectively. ELISA assay was used to examine the immunocompetence of each clone. 81. The concentration of serum Bone-specific Alkaline Phosphatase(BAP) and Tartrate-resistant Acid Phosphatase(TRAP) were measured by ELISA kits of IDS. 82. Conclusion This ELISA using recombinant OspC was a good method for early diagnostic of Lyme disease. 83. The MEIA and ELISA was used to detect tacrolimus valley point concentrations in heart, liver, small intestine and kidney transplantation recipients at different postoperative periods. 84. In this article, ELISA was applied to estimate 119 mother - babies blood in Anshan for HCMC - IgM. HCMV - IgA. 85. ELISA has merits of fast, sensitiveness and specificity to detected antibody of Mycobacterium paratuberculosis in serum of the cattle, the result was in accordance with that of clinical test. 86. Methods HPSA in stool of 62 patients were detected by ELISA, tissue samples silver stain. 87. The molecularly imprinted polymer prepared by the method has high selectivity and high specificity, can resist bad environments and can be coupled with ELISA. 88. Conclusion: PCR and ELISA techniques can be used for diagnosis of NTM infection. 89. Methods Data were collected through the on - the - spot investigation and serum specimens were tested by ELISA. 90. More than 25 human samples have proved positive for Rift Valley fever by PCR or ELISA testing.