plasmid造句
31. Methods The adhesion forces of MHCC97H cells with high metastatic potential transfected with sense or antisense KAI1 expression plasmid were measured by means of micropipette aspiration technique.
32. This indicates that S. griseus plasmid (s) codes streptidine phosphatase gene as well as some other involved in streptidine biosynthesis.
33. The recombinant plasmid pPGVT 3 derived from the vector pUC 4 was unstable in the E.
34. Methods: The gastric carcinoma cells had been successfully transfected with plasmid containing the C' distal end antisense sequence of glutaminase gene and called SGC-7901GA in previous researches.
35. AIM: To study the gene silencing effect of short hairpin plasmid vector on aquaporin-4 (AQP-4) in astrocytes cultured in vitro.
36. Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
37. The strains with recombinant plasmid of hEC - SOD were induced and expressed as inclusion body.
38. Agrobacterium method was used to transform the plasmid into Gerbera hybrida.
39. In 1973, the construction of the first biologically functional bacterial plasmid signaled the beginning of molecular cloning and recombinant DNA technology.
40. Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
41. Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.
42. Results:The constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
43. Church recalls seeing plasmid and gene sequences for the first time—penicillinase and insulin, the lac repressor and interferon.
44. We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
45. Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid.
46. The recombinant plasmid pcDNA3 F was stable within the host stain ZJ111 in vitro and in vivo as shown by restriction enzyme analysis and PCR identification of the F gene.
47. We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
48. Catechol 2,3-dioxygenase(C23O) gene was amplified with the specific primer and the template from plasmid in the strain ZL5 degrading phenanthrene and pyrene.
49. Results The recombinant plasmid with mutant gene was constructed correctly.
50. Lysosome and cellular plasmid are activated to restrain activity of heavy metals.
51. Objective To investigate the amelogenin expression of recombinant plasmid PcDNA 3 - AMG in COS - 1 cell line.
52. Conclusion: pED GCSF is a high eukaryotic expression plasmid in mammalian cell.
53. With common molecular biology technique, we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid.
54. Precomputed comparisons can be selected from a database covering all the completed bacterial chromosome and plasmid sequences in the Genome Reviews database.
55. Objective : To find a rapid method of screening positive recombinant plasmid.
56. The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.
57. The vitro methods include autoagglutination test, calcium dependency test, pyrazinamidase test, salicin fermentation, esculin hydrolysis, Congo red colony test and virulence plasmid.
58. The recombinant CYP 3 A 4 plasmid was successfully cloned and effectively expressed in Rosetta ( DE 3 ) 2 pLysS.
59. ObjectiveTo study the effect of eukaryotic express plasmid of augmenter of liver regeneration (ALR) on acute hepatic failure of rats.
60. Once a plasmid is snipped open, a foreign piece of DNA, cut by the same enzyme scissors, can be taped, end to end, into the plasmid using another enzyme, DNA ligase.
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