plasmid造句
61. Results The protein expressed by the plasmid which had a correct reading frame, had no transcriptional activation and could interact with a known coactivator PNRC.
62. This cut DNA is then mixed with the modified plasmid DNA, the two DNA strands join at their sticky ends and are sealed together by the enzyme, DNA ligase, to form a recombinant plasmid.
63. Methods We construct the rat antisense TIMP 1 recombinant plasmid which can be expressed in eucaryotic cells by RT Nest PCR and the technique of recombinant DNA.
64. Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor.
65. Objective To study primarily the safety of transfering recombinant human proinsulin gene plasmid(RHPGP)into diabetic rats induced by streptozotocin.
66. Objective : To survey plasmid profiles ( PP ) of Neisseria gonorrhoeae in Nanchang.
67. Objective To study the penicillin resistance in Neisseria gonorrhoeae isolates and genotypes of plasmid in penicillinase-producing N. gonorrhoeae isolates (PPNG) from Nanjing.
68. The factors influencing survival rate of LAB and plasmid stability during lyophilization were systematically studied.
69. Plasmid DNA profiles analysis offer scientific reference to molecular epidemiology investigation of avian colibacillosis .
70. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference, and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.
71. RAPD analysis had high distinguish ability and could provide more direct, rich and precise genetic information than plasmid profile analysis.
72. Finally a recombinant plasmid named pcNcSRS2, which was identified by PCR, restriction enzyme analysis and sequencing, was obtained.
73. Secreted proinsulin amounted to about 2 mg per liter was found in the culture medium of yeast strain 63 transformed by plasmid YTI-15.
74. Methods Isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method.
75. Aim a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
76. The result indicated, fed-batch culture could lead to the increase of thallus but without the increase of plasmid concentration.
77. ConclusionSuccessful HisTag-LRPAP1 construct encoding the fusion protein of the plasmid vector, the future can be used for eukaryotic expression of this fusion protein.
78. As a carrier system for gene therapy in animal model, VEGF-NP is much better than naked DNA plasmid. The results demonstrate great possibility of using NP carrier in human gene therapy.
79. The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
80. Objective To control the quality of mouse nerve growth factor ( NGF ) DNA plasmid for gene therapy.
81. The relationship between penicillin resistance and R plasmid was discussed.
82. Objective To construct the plasmid expressing BDV p 40 gene.
83. The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
84. Insert and ligate any DNA fragment into a vector such as plasmid or viral vector, to form a recombinant DNA.
85. Conclusions The recombinant plasmid constructed is useful for establishing a transgenic mouse.
86. Conclusion The recombinant plasmid that expressed the anti-human bladder carcinoma VH-PE38 immunotoxin is successfully obtained.
87. Then the recombinant plasmid was transfected into NK-92 cells with Hpofectamine encapsuled.
88. AIM: To construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP)-9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
89. By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.
90. Purified ATF - lys 10 specifically bound to uPAR - expressing cells and formed protein - DNA complexes with plasmid pGL 3 - control.
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