《中药生物技术》教学进度
教材: 中药生物技术,贾景明,专业教材,化学工业出版社,2008
教 学 内 容
(写明章、节扼要内容)
教学时数
教学方式
备注
实施方案、总论
2:
分组、评分规则、读书报告要求
中医学
2:
讲授讨论
阴阳表里五行
中药学
2:
讲授讨论
配伍:君臣佐使
次生代谢物
2:
分类结构合成途径
细胞工程
2:
喜树
细胞工程应用
2:
颠茄
发酵过程
2:
发酵应用
2:
功能红曲
生物转化应用
2:
基因的克隆与表达
2:
基因的克隆与表达应用
2:
花色苷、青蒿酸
基因技术
2:
基因技术与中药鉴定
2:
广藿香、鹿
作用机理
2:
作用机理应用
2:
茵陈蒿汤
机动
2:
复习
一,点评HW4
根据底物和产物的结构式确定酶的大类,也可以根据酶的编号。
需要注意的是合酶synthase与合成酶(即连接酶)synthetase/ligase
参考书
生物化学教程。王镜岩等。高等教育出版社。2008
几个酶的大类
5-磷酸脱氧木-酮糖还原酶1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR; EC 1.1.1.267),KM=250 μM for DXP
"Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues." Kuzuyama T., Takahashi S., Takagi M., Seto H. J. Biol. Chem. 275:19928-19932(2000)
https://www.uniprot.org/uniprot/P45568
色氨酸脱羧酶Tryptophan decarboxylase (TDC; EC 4.1.1.28), a pyridoxal dependent enzyme converts tryptophan to tryptamine.
栊牛儿醇-10羟化酶geraniol-10-hydroxylase (G10H; EC 2.5.1.1)
次番木鳖苷合酶secologanin synthase (SLS; EC 1.3.3.9)
异胡豆苷合酶strictosidine synthase (STR; EC 4.3.3.2)
"De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer’s properties" Guo, Q., Ma, X., Wei, S. et al. BMC Genomics (2014) 15: 676. https://doi.org/10.1186/1471-2164-15-676
secologanin synthase EC:1.14.19.62 "Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72A1 as secologanin synthase." Irmler S., Schroeder G., St Pierre B., Crouch N.P., Hotze M., Schmidt J., Strack D., Matern U., Schroeder J. Plant J. 24:797-804(2000)
二,授课
常用资源:
https://www.ncbi.nlm.nih.gov/
National Center for Biotechnology Information
http://www.sciencenet.cn/bbs/
科学网论坛
http://www.dxy.cn/
丁香园
http://www.bioon.com/
生物谷
http://www.ebiotrade.com/
生物通
http://www.genome.jp/
http://www.genome.jp/kegg/
KEGG Atlas Global maps of cell/organism functions
KEGG PATHWAY Pathway maps and pathway modules
KEGG BRITE Functional hierarchies and ontologies
KEGG GENES Genomes, genes, proteins, and orthologs
KEGG LIGAND Chemical compounds, drugs, glycans, and reactions
http://www.rcsb.org/pdb/home/home.do
The RCSB PDB provides a variety of tools and resources for studying the structures of biological macromolecules and their relationships to sequence, function, and disease.
杂交:Southern Blotting,Northern Blotting,Western Blotting
分子克隆/基因工程
区别:vector, carrier; selection, screening
三,作业(自由选择)
HW4四个酶的基因:name,ID,length
DXR,G10H,SLS,STR
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